报告人：江赐忠,博士,教授,博导,同济大学特聘教授

　　时间：2014年12月4日 下午1：30-2：30

　　会议室：中国科学院遗传与发育生物学研究所B210会议室

　　报告题目：Drosophila Brahma complex remodels nucleosome organizations in multiple aspects

　　摘要：

　　ATP-dependent chromatin remodeling complexes regulate nucleosome organizations. In Drosophila, gene Brm encodes the core Brahma complex, the ATPase subunit of SWI/SNF class of chromatin remodelers. Its role in modulating the nucleosome landscape in vivo is unclear. In this study, we knocked down Brm in Drosophila 3rd instar larvae to explore the changes in nucleosome profiles and global gene transcription. The results show that Brm knockdown leads to nucleosome occupancy changes throughout the entire genome with a bias in occupancy decrease. In contrast, the knockdown has limited impacts on nucleosome position shift. The knockdown also alters another important physical property of nucleosome positioning, fuzziness. Nucleosome position shift, gain or loss, and fuzziness changes are all enriched in promoter regions. Nucleosome arrays around the 5’ ends of genes are reorganized in five patterns as a result of Brm knockdown. Intriguingly, the concomitant changes in the genes adjacent to the Brahma-dependent remodeling regions have important roles in development and morphogenesis. Further analyses reveal abundance of AT-rich motifs for transcription factors in the remodeling regions.