报告题目：Characterization of gene fusions by Hybrid-Seq 

报告人：Kin Fai Au, University of Iowa, USA 

报告时间：3月9日下午14:00 

报告地点：1号楼B210会议室  

Dr. Kin Fai Au, Assistant Professor, Ph. D. (2004, The University of Oxford). Dr. Au’s group focuses on methodology research of Third Generation Sequencing (TGS) (especially for PacBio and Oxford Nanopore sequencing), with an emphasize on splicing, isoform construction, gene fusion and quantitative analysis. Currently, the Au lab is studying the transcriptome differences between embryonic stem cells and induced pluripotent stem cells. In addition, they are also developing statistical methods for integration of Mass Spec and sequencing data, as well as methodology research of gene regulatory network and RNA editing. The website of Dr. Au’s lab is http://www.healthcare.uiowa.edu/labs/au/ . 

Latest Publications 

1.Au KF*, Sebasiano V. (2014) The transcriptome of human pluripotent stem cells. Current Opinion in Genetics and Development. 28:71-77. (*Corresponding Author) 

2.Lo WS, Gardiner E, Xu Z, Lau CF, Wang F, Zhou JJ, Mendlein JD, Nangle LA, Chiang KP, Yang XL, Au KF, Wong WH, Guo M, Zhang M, Schimmel P. (2014) Human tRNA synthetase catalytic nulls with diverse functions. Science. 345 (6194):328-332. 

3.Qiu H, Lee S, Shang Y, Wang WY, Au KF, Kamiya S, Barmada SJ, Finkbeiner S, Lui H, Carlton CE, Tang AA, Oldham MC, Wang H, Shorter J, Filiano AJ, Roberson ED, Tourtellotte WG, Chen B, Tsai LH, Huang EJ. (2014) ALS-associated mutation FUS-R521C causes DNA damage and RNA splicing defects. The Journal of Clinical Investigation. 124(3):981-999. 

4.Au KF, Sebastiano V, Afshar PT, Durruthy JD, Lee L, Williams BA, Van Bakel H, Schadt EE, Reijo-Pera RA, Underwood JG, Wong WH. (2014) Characterization of the human ESC transcriptome by hybrid sequencing. Proc. Natl. Acad. Sci.U S A. 110(50):E4821-E4830. 

5.Tan MH*, Au KF*, Leong DE, Foygel K, Wong WH, Yao MW. (2013) An Oct4-Sall4-Nanog network controls developmental progression in the pre-implantation mouse embryo. Molecular Systems Biology. 9:632. (*These authors contributed equally to this work)