报告题目: Plant Genes Required for Immunity and Cell Death 报告人: Prof. John Mundy,Department of Biology, University of Copenhagen 主持人: 方荣祥院士 报告时间: 2010年11月15日（星期一）10:00-11:15 报告地点: 微生物所A203会议室

A class of Arabidopsis mutants exhibit lesions symptomatic of disease or accelerated cell death. The recessive acd11 mutant, caused by deletion of a sphingolipid trafficking protein, exhibits salicylate-dependent programmed cell death. ACD11 is not an essential gene as removal of SA by the SA hydroxylase NahG, or by mutations in the EDS1 or PAD4 genes required for SA signaling, suppress PCD in acd11. A PCD pathway with similar hormonal and genetic requirements is suppressed by mutations in autophagy (ATG) genes, as atg7 mutants are compromised in the SA-dependent hypersensitive response elicited by the avrRps4 effector, delivered by the bacterial pathogen P. syringae. To better understand such a pathway, suppressors of acd11-related death were isolated by survival after treatment with the non-metabolized SA agonist benzothiadiazole which induces PCD in acd11/nahG. The screens yielded suppressors representing genes in signaling for defense and PCD or effectors of PCD. Complementation analyses sorted alleles, and identified multiple alleles of PAD4 and EDS. This validated the utility of the screen. Four ‘novel’ suppressors were cloned and will be described

Brodersen et al. (2002) Knockout of Arabidopsis ACCELERATED-CELL-DEATH11 encoding a sphingosine transfer protein causes activation of programmed cell death & defense. Genes & Develop. 16, 490-502.

Brodersen et al. (2005) The role of salicylic acid in the induction of cell death in Arabidopsis acd11. Plant Physiol. 138, 1037-45.

Hofius et al. (2009) Autophagic Components Contribute to Hypersensitive Cell Death in Arabidopsis. Cell 137, 773-83.

Palma et al. (2010) Autoimmunity in Arabidopsis acd11 is mediated by epigenetic regulation of an immune receptor. PLOS Pathogens 6, e1001137.

Malinovsky et al. (2010) Lazarus1, a DUF300 Protein, Contributes to Programmed Cell Death Associated with Arabidopsis acd11 & the Hypersensitive Response. PLoS ONE 5(9): e12586. doi:10.1371/journal.pone.0012586.

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