Studies of protein-DNA interaction and gene regulation(报告时间:2013年8月30日15:00)

告题目:  Studies of protein-DNA interaction and gene regulation 
告人: 浩威 (多伦多大学化学与物理科学系) 
告时间:2013830 (周五)下午3 
告地点:遗传发育所B210 议室 
系人:降雨强 
系电话:64806362 

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Most of genes we know today are regulated by proteins bound to DNA. However, the complexity of interactions between protein/repressor and DNA is not been fully investigated yet. Instead of simply binding closely to a single DNA site, proteins separated thousands of basepairs away can still talk each other via protein-protein interaction and simultaneously change DNA geometry with DNA looping and wrapping. The formation of such protein-DNA complex significantly changes the dynamic of gene regulation and often serves as a key point of whole gene pattern.  

In this presentation, three different proteins (lambda repressor, 186 repressor and H-NS protein) and their unique method of protein-protein/protein-DNA interaction will be discussed. Modern single molecule techniques such as AFM (atomic force microscopy), optical tweezers and TPM (tethered particle motion) were used to obtain a better understanding on gene regulation in a biophysical view. 

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