Enhanced Seed Oil Production in Canola by Conditional Expression of Brassica napus LEAFY COTYLEDON1 (BnLEC1) and LEC1-LIKE (BnL1L) in Developing Seeds
| 作者： |
Helin Tan,Xiaohui Yang,Fengxia Zhang,Xiu Zheng,Cunmin Qu,Jinye Mu,Fuyou Fu,Jiana Li,Rongzhan Guan,Hongsheng Zhang,Guodong Wang and Jianru Zuo |
| 刊物名称： |
Plant Physiology |
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| 发表年度： |
2011-05-27 |
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| 摘要： |
The seed oil content in oilseed crops is a major selection trait to breeders. In Arabidopsis, LEAFY COTYLEDON1 (LEC1) and LEC1-LIKE (L1L) are key regulators of fatty acid biosynthesis. Overexpression of AtLEC1 and its orthologs in Brassica napus, BnLEC1 and BnL1L, causes an increased fatty acid level in transgenic Arabidopsis plants, which, however, also show severe developmental abnormalities. Here, we use truncated napin A promoters, which maintain the seed-specific expression pattern but with a reduced expression level, to drive the expression of BnLEC1 and BnL1L in transgenic canola. Conditional expression of BnLEC1 and BnL1L increases the seed oil content by 2~20%, and has no detrimental effects on major agronomic traits. In the transgenic canola, expression of a subset of genes involved in fatty acid biosynthesis and glycolysis is upregulated in developing seeds. Moreover, the BnLEC1 transgene enhances the expression of several genes involved in sucrose synthesis and transport in developing seeds and the silique wall. Consistently, the accumulation of sucrose and fructose is increased in developing seeds of the transgenic rapeseed, suggesting the increased carbon flux to fatty acid biosynthesis. These results demonstrate that BnLEC1 and BnL1L are reliable targets for genetic improvement of rapeseed in seed oil production. |
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