Lipidomic analysis of human tear fluid reveals structure-specific lipid alterations in dry eye syndrome
| 作者： |
Sin Man Lam,Louis Tong,Bastien Reux,Xinrui Duan,Andrea Petznick,Siew Sian Yong,Cynthia Boo Shiao Khee,Martin J. Lear,Markus R.. Wenk and Guanghou Shui |
| 刊物名称： |
J. Lipid Res |
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| 发表年度： |
2013-11-29 |
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| 摘要： |
As current diagnostic markers for dry eye syndrome (DES) are lacking in both sensitivity and specificity, a pressing concern exists to develop activity markers that closely align with the principal axes of disease progression. In this study, a comprehensive lipidomic platform designated for analysis of human tear lipidome was employed to characterize changes in tear lipid compositions from a cohort of 93 subjects of different clinical subgroups classified based on the presence of dry eye symptoms and signs. Positive correlations were observed between the tear levels of cholesteryl sulfates and glycosphingolipids with physiological secretion of tears, which indicated the possible lacrimal (instead of meibomian) origin of these lipids. Notably, we found wax esters of low molecular masses and those containing saturated fatty acyl moieties were specifically reduced with disease and significantly correlated with various DES clinical parameters such as ocular surface disease index, tear breakup time and Schirmers I test (i.e. both symptoms and signs). These structure-specific changes in tear components with DES could potentially serve as unifying indicators of disease symptoms and signs. In addition, the structurally-specific aberrations in tear lipids reported above were found in patients with or without aqueous-deficiency, suggesting a common pathology for both DES subtypes. |
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