Extensive characterization of human tear fluid collected using different techniques unravels the presence of novel lipid amphiphiles
| 作者： |
Sin Man Lam,Louis Tong,Xinrui Duan,Andrea Petznick,Markus R. Wenk and Guanghou Shui |
| 刊物名称： |
J. Lipid Res |
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| 发表年度： |
2013-11-29 |
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| 摘要： |
The tear film covers the anterior eye and the precise balance of its various constituting components is critical for maintaining ocular health. The composition of the tear film amphiphilic lipid sublayer, in particular, has largely remained a matter of contention due to the limiting concentrations of these lipid amphiphiles in tears that render their detection and accurate quantitation tedious. Using systematic and sensitive lipidomic approaches, we validated different tear collection techniques and report the most comprehensive human tear lipidome to date; comprising more than 600 lipid species from 17 major lipid classes. Our study confers novel insights to the compositional details of existent tear film model, in particular the disputable amphiphilic lipid sublayer constituents, by demonstrating the presence of cholesteryl sulfate, O-acyl-omega-hydroxy fatty acids, and various sphingolipids and phospholipids in tears. The discovery and quantitation of the relative abundances of various tear lipid amphiphiles reported herein are expected to have profound impact on the current understanding of the existent human tear film model. |
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