Tobacco TCTP interacts with ethylene receptor NTHK1 and enhances plant growth through promotion of cell proliferation
| 作者： |
Tao JJ,Cao YR,Chen HW,Wei W,Li QT,Ma B,Zhang WK,Chen SY and Zhang JS |
| 刊物名称： |
Plant Physiology |
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| 发表年度： |
2015-05-14 |
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| 摘要： |
Ethylene is an important phytohormone in the regulation of plant growth, development and stress response throughout the life cycle. Previously, we discovered that a subfamily II ethylene receptor NTHK1 from tobacco (Nicotiana tabacum) promotes seedling growth. Here, we identified a NTHK1-interacting protein NtTCTP (Nicotiana tabacum Translationally Controlled Tumor Protein) by the yeast two-hybrid assay and further characterized its roles in plant growth. The interaction was further confirmed by in vitro GST pull-down and in vivo Co-IP and BiFC assays, and the kinase domain of NTHK1 mediates the interaction with NtTCTP. The NtTCTP protein is induced by ethylene treatment, and co-localizes with NTHK1 at the endoplasmic reticulum. Overexpression of NtTCTP or NTHK1 reduces plant response to ethylene and promotes seedling growth mainly through acceleration of cell proliferation. Genetic analysis suggests that NtTCTP is required for the function of NTHK1. Furthermore, association of NtTCTP prevents NTHK1 from proteasome-mediated protein degradation. Our data suggest that plant growth inhibition triggered by ethylene is regulated by a novel feedback mechanism in which ethylene-induced NtTCTP associates with and stabilizes ethylene receptor NTHK1 to reduce plant response to ethylene and promote plant growth through acceleration of cell proliferation. |
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