Trophic Mode-Dependent Proteomic Analysis Reveals Functional Significance of Light-Independent Chlorophyll Synthesis in Synechocystis sp. PCC 6803
| 作者： |
Longfa Fang, Haitao Ge, Xiahe Huang, Ye Liu, Min Lu, Jinlong Wang, Weiyang Chen, Wu Xu, Yingchun Wang |
| 刊物名称： |
Molecular Plant |
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| 发表年度： |
2016-09-02 |
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| 摘要： |
The photosynthetic model organism Synechocystis sp. PCC 6803 can grow in different trophic modes depending on availability of light and exogenous organic carbon source. However, how the protein profile changes to allow the cells differentially propagate in different modes has not been comprehensively investigated. Using isobaric labeling based quantitative proteomics, we simultaneously identified and quantified 45% Synechocystis proteome across four different trophic modes, i.e., autotrophic, heterotrophic, photoheterotrophic, and mixotrophic modes. Among the 155 proteins that are differentially expressed across the trophic modes, proteins involved in nitrogen assimilation and light-independent chlorophyll synthesis are dramatically upregulated in the mixotrophic mode, and this is concomitant with dramatic increase of PII phosphorylation sensing carbon and nitrogen assimilation status. Moreover, functional study using a mutant defected in light-independent chlorophyll synthesis revealed that this pathway is important for chlorophyll accumulation under cycled light:dark illumination regime, a condition mimicking day:night cycles in certain natural habitats. Collectively, these results provide the most comprehensive information of trophic mode-dependent protein expression in the cyanobacterium, and for the first time, reveal the novel expression pattern and functional significance of light-independent chlorophyll synthesis in trophic growth. |
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