Upon perception by plant cells, the immunity hormone jasmonate (JA) triggers a genome-wide transcriptional program, which is largely regulated by the master transcription factor MYC2. The function of MYC2 depends on its physical and functional interaction with MED25, a subunit of the Mediator transcriptional co-activator complex. In addition to interacting with MYC2 and RNA polymerase II for preinitiation complex formation, MED25 also interacts with multiple genetic and epigenetic regulators and controls almost every step of MYC2-dependent transcription, including nuclear hormone receptor activation, epigenetic regulation, mRNA processing, transcriptional termination, and chromatin loop formation. These diversified functions have ascribed MED25 to a signal-processing and signal-integrating center during JA-regulated gene transcription. This review is focused on the interactions of MED25 with diverse transcriptional regulators and how these mechanistic interactions contribute to the initiation, amplification, and fine tuning of the transcriptional output of JA signaling.