Natural rubber (NR), valued for its elasticity and impact resistance, is essential for numerous industrial and medical applications, with global demand continuously rising. While approximately 2500 plant species from more than 40 families can produce rubber, the majority is sourced from Hevea brasiliensis grown in tropical regions. Alternative rubber-producing plants, such as Parthenium argentatum and Taraxacum kok-saghyz, offer enhanced environmental adaptability and species diversity, making them promising candidates for rubber production. Recent genome sequencing has shed light on rubber biosynthesis pathways, although the mechanisms involved in producing different forms of polyisoprene across species remain unclear. We explore the evolution of rubber biosynthesis and discuss synthetic biological strategies for enhancing NR-production in subtropical plants and a broader range of plant materials (e.g., Manilkara zapota).