论文--中国科学院遗传与发育生物学研究所

A Single-MYB Transcription Factor GmMYB331 Regulates Seed Oil Accumulation and Seed Size/Weight in Soybean

作者：	Zhouya Wang, Luyao Zhang, Bin Zhou, Jingjing Liang, Yanbao Tian, Zhihao Jiang, Jianjun Tao, Cuicui Yin, Shouyi Chen, Wanke Zhang, Jinsong Zhang and Wei Wei
刊物名称：	Journal of Integrative Plant Biology
DOI：
联系作者：
英文联系作者：
发布时间：	2025-12-11
卷：
摘要：	Seed oil accumulation is an important process affecting seed quality and regulatory factors modulating this process remain less understood especially in soybean. In this study, through RNA-seq and gene co-expression network analysis, we identified a single MYB (Myeloblastosis)-type transcription factor GmMYB331, which promotes seed oil accumulation in soybean seeds, and enhances seed size/weight as well. Transgenic soybean plants with mild GmMYB331 overexpression showed higher total fatty acid contents in seeds and higher seed yield per plant compared to the control plants. In contrast, transgenic soybean plants with strong GmMYB331 overexpression exhibited only increased seed size/weight but much reduced seed yield per plant, along with an altered plant architecture. Knocking out the GmMYB331 by CRISPR/Cas9 produced mutants with less total fatty acids, smaller seeds and less seed weight, indicating that the gene is required for oil accumulation and seed size/weight control. The GmMYB331 may achieve these functions by differential binding to the gene promoters and activation of the downstream genes, namely GmOLEO1/2/4 for oil accumulation in mild overexpressing plants and GmCYCD2;2 for seed size/weight increase in strong overexpressing plants. Our study reveals a possible mechanism involving differential regulation by GmMYB331 toward oil accumulation and seed size/weight increase. Manipulation of GmMYB331 gene may facilitate breeding for high oil and/or high yield soybean cultivars.
参与作者：