Rapid production of H₂O₂ is a hallmark of plant responses to diverse pathogens and plays a crucial role in signalling downstream of various receptors that perceive immunogenic patterns. However, mechanisms by which plants sense H₂O₂ to regulate immunity remain poorly understood. We show that endogenous H₂O₂ generated upon immune activation is sensed by the thiol peroxidase PRXIIB via oxidation at Cys51, and this is essential for stomatal immunity against Pseudomonas syringae. We further show that in immune-stimulated cells, PRXIIB conjugates via Cys51 with the type 2C protein phosphatase ABA insensitive 2 (ABI2), subsequently transducing H₂O₂ signal to ABI2. This oxidation dramatically sensitizes H₂O₂-mediated inhibition of the ABI2 phosphatase activity in vitro and is required for stomatal immunity in plants. Together, our results illustrate a redox relay, with PRXIIB as a sensor for H₂O₂ and ABI2 as a target protein, that mediates reactive oxygen species signalling during plant immunity.

Rapid production of H₂O₂ is a hallmark of plant responses to diverse pathogens and plays a crucial role in signalling downstream of various receptors that perceive immunogenic patterns. However, mechanisms by which plants sense H₂O₂ to regulate immunity remain poorly understood. We show that endogenous H₂O₂ generated upon immune activation is sensed by the thiol peroxidase PRXIIB via oxidation at Cys51, and this is essential for stomatal immunity against Pseudomonas syringae. We further show that in immune-stimulated cells, PRXIIB conjugates via Cys51 with the type 2C protein phosphatase ABA insensitive 2 (ABI2), subsequently transducing H₂O₂ signal to ABI2. This oxidation dramatically sensitizes H₂O₂-mediated inhibition of the ABI2 phosphatase activity in vitro and is required for stomatal immunity in plants. Together, our results illustrate a redox relay, with PRXIIB as a sensor for H₂O₂ and ABI2 as a target protein, that mediates reactive oxygen species signalling during plant immunity.

Rapid production of H₂O₂ is a hallmark of plant responses to diverse pathogens and plays a crucial role in signalling downstream of various receptors that perceive immunogenic patterns. However, mechanisms by which plants sense H₂O₂ to regulate immunity remain poorly understood. We show that endogenous H₂O₂ generated upon immune activation is sensed by the thiol peroxidase PRXIIB via oxidation at Cys51, and this is essential for stomatal immunity against Pseudomonas syringae. We further show that in immune-stimulated cells, PRXIIB conjugates via Cys51 with the type 2C protein phosphatase ABA insensitive 2 (ABI2), subsequently transducing H₂O₂ signal to ABI2. This oxidation dramatically sensitizes H₂O₂-mediated inhibition of the ABI2 phosphatase activity in vitro and is required for stomatal immunity in plants. Together, our results illustrate a redox relay, with PRXIIB as a sensor for H₂O₂ and ABI2 as a target protein, that mediates reactive oxygen species signalling during plant immunity.