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Histone H3.3 Deposition in Seed is Essential for the Post-Embryonic Developmental Competence in Arabidopsis
Ting Zhao, Jingyun Lu, Huairen Zhang, Mande Xue, Jie Pan, Lijun Ma, Frédéric Berger & Danhua Jiang
Nature Communications
Abstract
The acquisition of germination and post-embryonic developmental ability during seed maturation is vital for seed vigor, an important trait for plant propagation and crop production. How seed vigor is established in seeds is still poorly understood. Here, we report the crucial function of Arabidopsis histone variant H3.3 in endowing seeds with post-embryonic developmental potentials. H3.3 is not essential for seed formation, but loss of H3.3 results in severely impaired germination and post-embryonic development. H3.3 exhibits a seed-specific 5′ gene end distribution and facilitates chromatin opening at regulatory regions in seeds. During germination, H3.3 is essential for proper gene transcriptional regulation. Moreover, H3.3 is constantly loaded at the 3′ gene end, correlating with gene body DNA methylation and the restriction of chromatin accessibility and cryptic transcription at this region. Our results suggest a fundamental role of H3.3 in initiating chromatin accessibility at regulatory regions in seed and licensing the embryonic to post-embryonic transition.
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论文编号: |
DOI: 10.1038/s41467-022-35509-6 |
论文题目: |
Histone H3.3 Deposition in Seed is Essential for the Post-Embryonic Developmental Competence in Arabidopsis |
英文论文题目: |
Histone H3.3 Deposition in Seed is Essential for the Post-Embryonic Developmental Competence in Arabidopsis |
第一作者: |
Ting Zhao, Jingyun Lu, Huairen Zhang, Mande Xue, Jie Pan, Lijun Ma, Frédéric Berger & Danhua Jiang |
英文第一作者: |
Ting Zhao, Jingyun Lu, Huairen Zhang, Mande Xue, Jie Pan, Lijun Ma, Frédéric Berger & Danhua Jiang |
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2022-12-15 |
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The acquisition of germination and post-embryonic developmental ability during seed maturation is vital for seed vigor, an important trait for plant propagation and crop production. How seed vigor is established in seeds is still poorly understood. Here, we report the crucial function of Arabidopsis histone variant H3.3 in endowing seeds with post-embryonic developmental potentials. H3.3 is not essential for seed formation, but loss of H3.3 results in severely impaired germination and post-embryonic development. H3.3 exhibits a seed-specific 5′ gene end distribution and facilitates chromatin opening at regulatory regions in seeds. During germination, H3.3 is essential for proper gene transcriptional regulation. Moreover, H3.3 is constantly loaded at the 3′ gene end, correlating with gene body DNA methylation and the restriction of chromatin accessibility and cryptic transcription at this region. Our results suggest a fundamental role of H3.3 in initiating chromatin accessibility at regulatory regions in seed and licensing the embryonic to post-embryonic transition. |
英文摘要: |
The acquisition of germination and post-embryonic developmental ability during seed maturation is vital for seed vigor, an important trait for plant propagation and crop production. How seed vigor is established in seeds is still poorly understood. Here, we report the crucial function of Arabidopsis histone variant H3.3 in endowing seeds with post-embryonic developmental potentials. H3.3 is not essential for seed formation, but loss of H3.3 results in severely impaired germination and post-embryonic development. H3.3 exhibits a seed-specific 5′ gene end distribution and facilitates chromatin opening at regulatory regions in seeds. During germination, H3.3 is essential for proper gene transcriptional regulation. Moreover, H3.3 is constantly loaded at the 3′ gene end, correlating with gene body DNA methylation and the restriction of chromatin accessibility and cryptic transcription at this region. Our results suggest a fundamental role of H3.3 in initiating chromatin accessibility at regulatory regions in seed and licensing the embryonic to post-embryonic transition. |
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Nature Communications |
英文刊物名称: |
Nature Communications |
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