--中国科学院遗传与发育生物学研究所

姓　　名：	肖军
职　　称：	研究员
职　　务：
电话/传真：
电子邮件：	jxiao@genetics.ac.cn
实验室主页：
研究方向：	作物功能基因组学

简历介绍：

肖军博士，研究员，博士生导师

教育经历
2006.09-2012.12	中国科学院植物研究所获博士学位
2002.09-2006.06	上海交通大学获学士学位

工作经历
2019.04-至今	John Innes Center-CAS 联合中心（CEPAMS）课题组长
2018.06-至今	中国科学院遗传与发育生物学研究所研究员
2013.07-2018.05	美国宾夕法尼亚大学博士后
2013.02-2013.06	中国科学院遗传与发育生物学研究所博士后

2023年获北京市“杰出青年基金”。长期从事表观遗传调控植物生长发育及对外部环境变换的适应性相关研究。近三年来以小麦为研究材料，在产量性状和养分高效相关研究取得一系列进展，以（共同）通讯作者在Nature、Nature Plants、Molecular Plant、Nature Communications、Genome Biology等发表论文12篇，受邀为Science China Life Science、Trends in Plant Science撰写综述。目前担任Faculty 1000独立评论员，Journal of Genetics and Genomics、Scientific Reports杂志（青年）编委，中国植物学会植物整合组学专委会委员。

奖励荣誉

2023年	北京市自然科学基金委“杰出青年基金”
2023年	中国作物学会科学技术成就奖-青年奖
2023年	中国科学院遗传与发育生物学研究所“益海嘉里优秀导师奖”
2017年	ICAR Travel Awards
2016年	Keystone Symposia Scholarship
2016年”	中国科学院百篇“优秀博士论文

研究领域：

小麦产量性状和养分高效的遗传基础解析及新种质创制

小麦是全球主要的粮食作物之一，为约40%的世界人口提供口粮。我们以小麦为材料，综合运用分子遗传学、基因组学、表观遗传学、生物化学和基因编辑技术等策略，就小麦产量相关组织器官（穗和种子）发育的重要调控因子进行系统挖掘和功能研究，解析影响小麦节水以及氮素高效利用的遗传调控网络，挖掘重要因子及优异等位变异，结合分子标记辅助选择、基因精准编辑等技术，应用于小麦稳产、氮素高效性状的种质创制和育种改良。

图1.多维组学整合分析系统、高效鉴定小麦复杂农艺性状相关因子

基因组三维结构对小麦驯化及农艺性状演化的调控

染色质的构象有序且对于正确执行生物活动至关重要。在不同的环境和发育条件下，染色质会发生全局和局部的重新排列，伴随着基因表达模式的改变。借助Hi-C,Ocean-C等技术，我们旨在研究小麦在多倍体化和驯化过程中，以及适应多变环境时的3D染色质结构动态。结合表观基因组学，如组蛋白修饰、DNA甲基化、染色质的开
放/关闭区域，以及转录组学，如长链非编码RNA和mRNA测序，以及特定因子的结合剖析（ChIP-seq），深入地了解染色质3D结构的形成、维持、变化和功能。

图2.染色质三维结构及表观组学分析促进小麦农艺性状演化调控解析

作物再生机理解析及应用

作物基因功能研究和品种改良离不开遗传转化。相较于拟南芥中简单的“滴花转化”的遗传转化方式，小麦等作物中需要长时间的组织培养才能获得遗传转化植株，且效率低，具有很强的品种依赖性，以及受限于特定的外植体。我们将借助多维组学分析，探究小麦再生过程的转录和染色质动态变化，搭建层级调控网络，解析小麦再生过程不同环节的作用机理；鉴定促进愈伤诱导及分化的因子，借助化学抑制剂及物理处理，提高小麦不同品种的转化效率；同时拓展外植体的选择，简化并优化再生过程的操作；同时将优化的再生流程拓展到禾本科其他作物以及重要的战略资源植物，推动基因功能解析及优异品种的培育。

图3.小麦幼胚再生过程的层级调控网络及染色质动态变化

学生培养情况简介：

已培养硕士1名，博士3名。1名美国HHMI实验室博士后，1名国内国家实验室博士后，1名国内省重点实验室青年研究员，1名国内生物行业龙头企业就职。

学生多人次获国家奖学金，益海嘉里优秀博士生，地奥奖学金，三好学生标兵；出访美国、英国、澳大利亚、日本等参加国际会议，做口头汇报。

近期招聘信息：

本实验室目前计划招聘助理/副研究员1名（岗位聘用），研究助理1名（项目聘用）和客座研究生1-2名，欢迎对表观遗传调控、小麦产量及氮素高效研究感兴趣的青年学子申请。

社会任职：

获奖及荣誉：

承担科研项目情况：

代表论著：

发表文章 (*Corresponding author; #Co-first author)

https://scholar.google.com/citations?user=XBQFE5IAAAAJ&hl=en

2023

1.Jinchao Chen#, Long Zhao#, Haoran Li#, Changfeng Yang, Dongzhi Wang, Xuelei Lin, Yujing Lin, Hao Zhang, Xiaomin Bie, Peng Zhao, Shengbao Xu, Xiansheng Zhang, Xueyong Zhang, Yingyin Yao, Jun Xiao*. (2023) TaNF-Y-PRC2 orchestrates temporal control of starch and protein synthesis in wheat. bioRxiv 2023.12.26.573020; doi: https://doi.org/10.1101/2023.12.26.573020

2.Xuemei Liu#, Xuelei Lin#*, Min Deng#, Bingxin Shi, Jinchao Chen, Haoran Li, Shujuan Xu, Xiaomin Bie, Xiansheng Zhang, Kang Chong, Jun Xiao*. (2023) Distinct roles of H3K27me3 and H3K36me3 in vernalization response, maintenance and resetting in winter wheat. bioRxiv 2023.12.19.572364; doi: https://doi.org/10.1101/2023.12.19.572364

3.Hao Zhang, Jinyuan Shi, Fa Cui, Long Zhao, Xiaoyu Zhang, Jinchao Chen, Jing Zhang, Yongpeng Li, Yanxiao Niu, Lei Wang, Caixia Gao, Xiangdong Fu, Yiping Tong, Hong-Qing Ling*, Junming Li*, Jun Xiao*. (2023) Epigenetic modifications mediate cultivar-specific root development and metabolic adaptation to nitrogen availability in wheat. Nat Commun. 2023 Dec 12;14(1):8238. doi: 10.1038/s41467-023-44003-6.

4.Zhang J, Zhang Z, Zhang R, Yang C, Zhang X, Chang S, Chen Q, Rossi V, Zhao L, Xiao J, Xin M, Du J, Guo W, Hu Z, Liu J, Peng H, Ni Z, Sun Q, Yao Y. Type I MADS-box transcription factor TaMADS-GS regulates grain size by stabilizing cytokinin signalling during endosperm cellularization in wheat. Plant Biotechnol J. 2023 Sep 26. doi: 10.1111/pbi.14180. Epub ahead of print. PMID: 37752705.

5.Hao Zhang, Xiaoyu Zhang, Jun Xiao*. Epigenetic regulation of nitrogen signaling and adaptation in plants. Plants, 2023, 12(14), 2725; https://doi.org/10.3390/plants12142725

6.Xiaowei Lin*, Tingting Yuan, Hong Guo, Yi Guo, Nobutoshi Yamaguchi, Shuge Wang, Dongxia Zhang, Dongmei Qi, Jiayu Li, Qiang Chen, Xinye Liu, Long Zhao, Jun Xiao, Doris Wagner, Sujuan Cui*, Hongtao Zhao*. The regulation of chromatin configuration at AGAMOUS locus by LFR-SYD-containing complex is critical for reproductive organ development in Arabidopsis. The Plant Journal, https://doi.org/10.1111/tpj.16385

7.Xuemei Liu#, Xiaomin Bie#, Xuelei Lin#, Menglu Li, Hongzhe Wang, Xiaoyu Zhang, Yiman Yang, Chunyan Zhang, Xiansheng Zhang*, Jun Xiao*. (2022) Uncovering transcriptional regulatory network during regeneration for boosting wheat transformation. Nat Plants. 2023 May 4. doi: 10.1038/s41477-023-01406-z. Epub ahead of print. PMID: 37142750.

8.Dongzhi Wang#, Yongpeng Li#, Haojie Wang#, Yongxin Xu#, Yiman Yang#, Yuxin Zhou, Zhongxu Chen, Yuqing Zhou, Lixuan Gui, Chunjiang Zhou, Wenqiang Tang, Shuzhi Zheng, Lei Wang, Xiulin Guo, Yingjun Zhang, Fa Cui, Xuelei Lin, Yuling Jiao, Yuehui He, Junming Li*, Fei He*, Xigang Liu*, Jun Xiao* (2022) Boosting wheat functional genomics via indexed EMS mutant library of KN9204. Plant Commun. 2023 Mar 20:100593. doi: 10.1016/j.xplc.2023.100593.

9.Xuelei Lin#, Yongxin Xu#, Dongzhi Wang#, Yiman Yang, Xiaoyu Zhang, Xiaomin Bie, Hongzhe Wang, Jiafu Jiang, Zhongxu Chen, Lixuan Gui, Yiliang Ding, Yuqing Che, Aili Li, Xueyong Zhang, Fei Lu, Xiangyu Zhao, Xiansheng Zhang, Xiangdong Fu, Jun Xiao*. (2023) Systematic mining and functional analysis of factors regulating wheat spike development for breeding selection. BioRxiv doi: https://doi.org/10.1101/2022.11.11.516122

10. Xumei Luo#, Yiman Yang#, Xuelei Lin*, Jun Xiao*. Decipherign spike architecture formation towards yield improvement in wheat. J Genet Genomics. 2023 Mar 10:S1673-8527(23)00054-1. doi: 10.1016/j.jgg.2023.02.015.

11. Long Zhao*, Yiman Yang, Jinchao Chen, Xuelei Lin, Hao Zhang, Hao Wang, Hongzhe Wang, Xiaomin Bie, Jiafu Jiang, Xiaoqi Feng, Xiangdong Fu, , Xiansheng Zhang, Zhuo Du, Jun Xiao*. Dynamic chromatin regulatory programs during embryogenesis of hexaploid wheat. Genome Biology, 2023 Jan 13;24(1):7. doi: 10.1186/s13059-022-02844-2.

12. Xuemei Liu#*, Kehui Zhu#, Jun Xiao*. Recent advances in understanding of the epigenetic regulation of plant regeneration. aBIOTECH, 2023 https://doi.org/10.1007/s42994-022-00093-2

2022

1.Jun Xiao*, Bao Liu, Yingyin Yao, Zifeng Guo, Haiyan Jia, Lingrang Kong, Aimin Zhang, Wujun Ma, Zhongfu Ni, Shengbao Xu, Fei Lu, Yuannian Jiao, Wuyun Yang, Xuelei Lin, Silong Sun, Zefu Lu, Lifeng Gao, Guangyao Zhao, Shuanghe Cao, Qian Chen, Kunpu Zhang, Mengcheng Wang, Meng Wang, Zhaorong Hu, Weilong Guo, Guoqiang Li, Xin Ma, Junming Li, Fangpu Han, Xiangdong Fu, Zhengqiang Ma, Daowen Wang*, Xueyong Zhang*, Hong-Qing Ling*, Guangmin Xia*, YipingTong*, Zhiyong Liu*, Zhonghu He*, Jizeng Jia*, Kang Chong*. Wheat genomic study for genetic improvement of traits in China. Science China Life Science. 2022 August, 65: 1718-1775. https://www.sciengine.com/SCLS/doi/10.1007/s11427-022-2178-7.

2.Xiaoli Shi#, Fa Cui#, Xinyin Han#, Yilin He#, Long Zhao#, Na Zhang#, Hao Zhang#, Haidong Zhu, Zhexin Liu, Bin Ma, Shusong Zheng, Wei Zhang, Jiajia Liu, Xiaoli Fan, Yaoqi Si, Shuiquan Tian, Jianqing Niu, Huilan Wu, Xuemei Liu, Zhuo Chen, Deyuan Meng, Xiaoyan Wang, Liqiang Song, Lijing Sun, Jie Han, Hui Zhao, Jun Ji, Zhiguo Wang, Xiaoyu He, Ruilin Li, Xuebin Chi, Chengzhi Liang*, Beifang Niu*, Jun Xiao*, Junming Li*, HongQing Ling*. Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen use efficiency in the wheat cultivar Kenong 9204. Mol Plant. 2022. https://doi.org/10.1016/j.molp.2022.07.008

3.Shengnan Li#, Dexing Lin#, Yunwei Zhang#, Min Deng#, Yongxing Chen, Bin Lv, Boshu Li, Yuan Lei, Yanpeng Wang, Long Zhao, Yueting Liang. Jinxing Liu, Kunling Chen, Zhiyong Liu, Jun Xiao*, Jinlong Qiu*, Caixia Gao*. Genome edited powdery mildew resistance in wheat without growth penalties, Nature. 2022 Feb;602(7897):455-460. doi: 10.1038/s41586-022-04395-9.

4.Un-Sa Lee, Tomasz Bieluszewski, Jun Xiao, Ayako Yamaguchi, Doris Wanger. (2022) H2A.Z contributes to Trithorax activity at the AGAMOUS locus. Mol Plant. https://doi.org/10.1016/j.molp.2022.01.005

5.Tingting Yang, Dingyue Wang, Guanmei Tian, Linhua Sun, Minqi Yang, Xiaochang Yin, Jun Xiao, Yu Sheng, Danmeng Zhu, Hang He, Yue Zhou*. Chromatin remodeling complexes regulate genome architecture in Arabidopsis. Plant Cell. 2022 Jul 4;34(7):2638-2651. doi: 10.1093/plcell/koac117. PMID: 35445713; PMCID: PMC9252501.

6.Yanmin Chen, Jun Xiao, Peng Liu. Dissecting the plant chromatin interactome using mass spectrometry. Trends Biotechnol. 2022 Mar;40(3):261-265. doi: 10.1016/j.tibtech.2021.06.002.

2021

1.Tomasz Bieluszewski#, Jun Xiao#, Yiman Yang, Doris Wagner. (2021) PRC2 activity, recruitment, and silencing: a comparative perspective. Trends Plant Sci. 2021 Jul 19:S1360-1385(21)00148-5. doi: 10.1016/j.tplants.2021.06.006. Epub ahead of print. PMID: 34294542.

2.Shujuan Xu, Qi Dong, Min Deng, Dexing Lin, Jun Xiao, Peilei Cheng, Lijing Xing, Yuda Niu, Caixia Gao, Wenhao Zhang, Yunyuan Xu, Kang Chong.(2021) The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat. Mol Plant. 2021 Sep 6;14(9):1525-1538. doi: 10.1016/j.molp.2021.05.026. Epub 2021 May 27. PMID: 34052392.

3.Run Jin#, Samantha Klasfeld#, Yang Zhu, Meilin Fernandez Garcia, Jun Xiao, Soon-Ki Han, Adam Konko, Doris Wagner. LEAFY is a pioneer transcription factor and licenses cell reprogramming to floral fate. Nature Communications, 2021 12(1):626. doi: 10.1038/s41467-020-20883-w.

2019

1.Bo Sun, Yingying Zhou, Jie Cai, Erlei Shang, Nobutoshi Yamaguchi, Jun Xiao, Liang-Sheng Looi, Wan-Yi Wee, Xiuying Gao, Doris Wagner, Toshiro Ito. Integration of transcriptional repression and Polycomb-mediated silencing of WUSCHEL in floral meristems. Plant Cell 2019. DOI: https://doi.org/10.1105/tpc.18.00450.

2.Shujuan Xu, Jun Xiao, Fang Yin, Xiaoyu Guo, Lijing Xing, Yunyuan Xu, Kang Chong. The protein modifications of O-GlcNAcylation and phosphorylation mediate vernalization response for flowering in winter wheat. Plant Physiology,2019. DOI: https://doi.org/10.1104/pp.19.00081.

2018 and before (PhD and Post-doc work)

1.Xiao J, Jin R, Yu X, Shen M, Wagner J, Pai A, Song C, Zhuang M, Kelasfeld S, He CS, Santos AM, Helliwell C, Pruneda-Paz J, Kay S, Lin XW, Cui SJ, Fernandez M, Clarenz O, Goodrich J, Zhang XY, Austin R, Bonasio R, Wagner D. (2017) Cis- and trans-determinants of Polycomb Repressive Complex 2 recruitment in Arabidopsis. Nature Genetics, doi:10.1038/ng.3937 (Nature Genetic news and views highlight, F1000 Prime)

2.Xiao J, Xu S#, Li C#, Xu Y, Xing L, Niu Y, Huan Q, Tang Y, Zhao C, Wagner D, Gao C, Chong K. (2014) O-GlcNAc-mediated interaction between VER2 and TaGRP2 elicits TaVRN1 mRNA accumulation during vernalization in winter wheat. Nature Communications 5:4572. (F1000 Prime)

3.Wu MF#, Yamaguchi N#, Xiao J#, Bargmann B, Estelle M, Sang Y, Wagner D. (2015) An auxin-regulated chromatin state switch directs organogenesis in Arabidopsis. eLife 4:e09269. (Co-first author, F1000 Prime)

4.Xiao J#, Jin R#, Wagner D. (2017) Developmental transitions: integrating environmental cues with hormonal signaling in the chromatin landscape. Genome Biology, 18 (1):88.

5.Xiao J, Wagner D. (2015) Polycomb repression in the regulation of growth and development in Arabidopsis. Curr Opin Plant Biol 23C:15-24.

6.Xiao J#, Li CH#, Xu SJ, Xing LJ, Xu YY, Chong K. (2015) AtJAC1 Regulates Nuclear Accumulation of GRP7, influencing RNA processing of FLC antisense transcripts and flowering time in Arabidopsis. Plant Physiol 169 (3):2102-2117.

7.Xiao J, Lee US, Wagner D. (2016) Tug of war: adding and removing histone lysine methylation in Arabidopsis. Curr Opin Plant Biol 4:41-53.

8.Xiao J, Zhang H, Xing LJ, Xu SJ, Liu HH, Chong K, Xu YY. (2013) Requirement of histone acetyltransferases HAM1/2 for epigenetic modification of FLC to regulate flowering in Arabidopsis. J Plant Physiol 170(4):444-451.

9.Xing LJ, Liu Y, Xu SJ, Xiao J, Wang B, Deng HW, Lu Z, Xu YY, Chong K. (2018) Arabidopsis O-GlcNAc transferase SEC actives histone methyltransferase ATX1 to regulate flowering. EMBO J, doi:10.15252/embj.201798115.

10. Ma Y, Dai X, Xu Y, Luo W, Zheng X, Zeng D, Pan Y, Lin X, Liu H, Zhang D, Xiao J, Guo X, Xu S, Niu Y, Jin J, Zhang H, Xu X, Li L, Wang W, Qian Q, Ge S, Chong K. (2015) COLD1 Confers Chilling Tolerance in Rice. Cell 160(6):1209-1221. (F1000 Prime, Cover story)

11. Bossi F, Fan J, Xiao J, Chandra L, Shen M, Wagner D, Rhee SY. (2017) Systematic discovery of novel eukaryotic transcriptional regulators using non-sequence homology based prediction. BMC Genomics, 18:480 DOI: 10.1186/s12864-017-3853-9

12. Liu YJ, Xu YY, Xiao J, Ma QB, Li D, Xue Z, Chong K. (2011) OsDOG, a gibberellin-induced A20/AN1 zinc-finger protein, negatively regulates gibberellin-mediated cell elongation in rice. J Plant Physiol 168(10):1098-1105.

13. Li J, Jiang JF, Qian Q, Xu YY, Zhang C, Xiao J, Du C, Luo W, Zou GX, Chen ML, Huang YQ, Feng YQ, Cheng ZK, Yuan M, Chong K. (2011) Mutation of Rice BC12/GDD1, Which Encodes a Kinesin-Like Protein That Binds to a GA Biosynthesis Gene Promoter, Leads to Dwarfism with Impaired Cell Elongation. Plant Cell 23(2):628-640.

14. Ma QB, Dai XY, Xu YY, Guo J, Liu YJ, Chen N, Xiao J, Zhang DJ, Xu ZH, Zhang XS* and Chong K*. (2009) Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes. Plant Physiol 150: 244-256.

软件著作权及专利

2023SR1031506

Find. Cis. Regulatory. Element. Target软件【简称：FCT软件】V1.0

HC221882 TaRF1基因及其编码的蛋白在提高小麦转化效率中的应用（3/7）

HC221883 TaDOF5.6基因在小麦高效遗传转化的应用（4/7）