--中国科学院遗传与发育生物学研究所

姓　　名：	何康敏
职　　称：	研究员
职　　务：
电话/传真：	010-6480-6564
电子邮件：	kmhe@genetics.ac.cn
实验室主页：
研究方向：	囊泡运输和信号脂质的时空动态、调控与疾病

简历介绍：

何康敏，博士，研究员，博士生导师

2008年在北京大学医学部获得学士学位，2013年在北京大学获得生物力学与医学工程博士学位。2013年至2019年在哈佛医学院/波士顿儿童医院先后从事博士后、Instructor/Research Associate研究，2019年加入中国科学院遗传与发育生物学研究所。近年来以通讯或共通讯作者在Nature、Cell、Nature Cell Biology、Molecular Cell等杂志发表多篇科研论文。

研究领域：

研究方向：

哺乳动物细胞含有近千种脂质分子，除了作为膜结构的基本组成部分和参与能量储存，一些脂质分子还可作为信号分子参与和调控细胞的信号转导、囊泡运输、细胞器功能、细胞迁移和增殖等各种生命活动过程，脂质的代谢和分布紊乱与多种人类重大疾病例如肿瘤的发生发展密切相关。

我们利用活细胞单分子荧光成像、图像定量分析、脂质分子探针开发、基因编辑等技术以及发展新的细胞生物学工具，通过多学科交叉的研究手段，探索脂质分子在细胞的囊泡运输、信号转导和肿瘤发生发展中的作用和调控机制。

1. 解析脂质分子的时空动态、功能与调控机制；

2. 揭示脂质分子在肿瘤发生发展中的作用和机制；

3. 发现细胞囊泡运输的新途径和新机制。

本实验室常年招聘博士后和实习生，诚挚欢迎有志于从事生命科学研究，对利用多学科交叉技术开展创新型研究感兴趣的伙伴，加入我们的队伍。

社会任职：

获奖及荣誉：

承担科研项目情况：

代表论著：

代表性论文（*Corresponding author;^#Co-first author）：

Jiachao Xu^#, Yu Liang^#, Nan Li^#, Song Dang^#, Amin Jiang, Yiqun Liu, Yuting Guo, Xiaoyu Yang, Yaran Yang, Yongtao Du, Yi Yuan, Xinyi Zhang, Anbing Shi, Xiaoyun Liu, Dong Li, Kangmin He*. Clathrin-associated carriers enable recycling via a kiss-and-run mechanism. Nature Cell Biology (2024) 26:1652–1668.

Nat Cell Biol | 何康敏团队揭示新的囊泡循环机制

https://mp.weixin.qq.com/s/42z8KI_FQryyDYcw1So4Ew

Jing Wang^#, Zhengyang An^#, Zhongsheng Wu^#, Wei Zhou^#, Pengyu Sun, Piyu Wu, Song Dang, Rui Xue, Xue Bai, Yongtao Du, Rongmei Chen, Wenxu Wang, Pei Huang, Sin Man Lam, Youwei Ai, Suling Liu, Guanghou Shui, Zhe Zhang, Zheng Liu, Jianyong Huang, Xiaohong Fang*, Kangmin He*. Spatial organization of PI3K-PI(3,4,5)P₃-AKT signaling by focal adhesions. Molecular Cell (accepted).

Zhongsheng Wu^#, Yongtao Du^#, Tom Kirchhausen*, Kangmin He*. Probing and imaging phospholipid dynamics in live cells. Life Metabolism (2024) 4(3), loae014.

Life Metabolism | 何康敏和Tom Kirchhausen团队合作发表活细胞磷脂动态的探测和成像的综述文章

https://mp.weixin.qq.com/s/Nr4hCU8-v90gK15e2Y7yDQ

Yue Miao^#, Yongtao Du^#, Baolei Wang, Jingjing Liang, Yu Liang, Song Dang, Jiahao Liu, Dong Li, Kangmin He*, Mei Ding*. Spatiotemporal recruitment of the ubiquitin-specific protease USP8 directs endosome maturation. eLife (2024). https://doi.org/10.7554/eLife.96353.2.

Jiachao Xu, Han Xiao, Kangmin He*, Youyi Zhang*. Crosstalk between adrenergic receptors and catalytic receptors. Current Opinion in Physiology (2023) 36:100718.

Xue Bai^#, Pengyu Sun^#, Xinghao Wang^#, Changkun Long^#, Shuyun Liao^#, Song Dang, Shangshang Zhuang, Yongtao Du, Xinyi Zhang, Nan Li, Kangmin He*, Zhe Zhang*. Structure and dynamics of the EGFR/HER2 heterodimer. Cell Discovery (2023) 9(1):18.

Yi Yuan^#, Xinghao Wang^#, Jie Jin, Zhiheng Tang, Wei Xian, Xinyi Zhang, Jiaqi Fu*, Kangmin He*, Xiaoyun Liu*. The Salmonella Typhimurium effector SpvB subverts host membrane trafficking by targeting clathrin and AP-1. Molecular & Cellular Proteomics (2023) 22(12):100674.

Guozhi Bi^#, Min Su^#, Nan Li^#, Yu Liang, Song Dang, Jiachao Xu, Meijuan Hu, Jizong Wang, Minxia Zou, Yanan Deng, Qiyu Li, Shijia Huang, Jiejie Li, Jijie Chai*, Kangmin He*, Yu-Hang Chen*, Jian-Min Zhou*. The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling. Cell (2021) 184:3528-3541.e12.

Cell突破！周俭民/陈宇航/何康敏/柴继杰合作揭示ZAR1抗病小体激活免疫反应的分子机制

https://mp.weixin.qq.com/s/opqpkudNZDXBSMHtZrjpnQ

Kangmin He^#, Eli Song^#, Srigokul Upadhyayula^#, Song Dang, Raphael Gaudin, Wesley Skillern, Kevin Bu, Benjamin R. Capraro, Iris Rapoport, Ilja Kusters, Minghe Ma, Tom Kirchhausen. Dynamics of Auxilin 1 and GAK in clathrin-mediated traffic. Journal of Cell Biology (2020) 219(3) pii: e201908142.

Kangmin He*, Robert Marsland III, Srigokul Upadhyayula, Eli Song, Song Dang, Ben Capraro, Weiming Wang, Wesley Skillern, Raphael Gaudin, Minghe Ma, Tom Kirchhausen*. Dynamics of phosphoinositide conversion in clathrin-mediated endocytic traffic. Nature (2017) 552:410-414.

Nan Li, Rong Zhao, Yahong Sun, Zi Ye, Kangmin He*, Xiaohong Fang*. Single-molecule imaging and tracking of molecular dynamics in living cells. National Science Review (2017) 4:739–760.

Kangmin He, Xiaohua Yan, Nan Li, Song Dang, Li Xu, Bing Zhao, Zijian Li, Zhizhen Lv, Xiaohong Fang*, Youyi Zhang*, Ye-Guang Chen*. Internalization of the TGF-β type I receptor into caveolin-1 and EEA1 double-positive early endosomes. Cell Research (2015) 25:738-752.

Other Publications:

Songzi Liu^#, Xiaoge Zhang^#, Xin Yao^#, Guan Wang, Shijia Huang, Peng Chen, Mingliang Tang, Jie Cai, Zhuyin Wu, Yiliang Zhang, Rongzhi Xu, Kai Liu, Kangmin He, Yan Wang, Lei Jiang, Qiong A Wang, Liangyou Rui, Jianmiao Liu, Yong Liu*. Mammalian IRE1α dynamically and functionally coalesces with stress granules. Nature Cell Biology (2024) 26(6): 917-931.

Tiantian Ma^#*, Yaqing Wang^#, Laikang Yu, Jinghua Liu, Tao Wang, Pengyu Sun, Yinghang Feng, Dan Zhang, Lei Shi, Kangmin He, Li Zhao, Zhiheng Xu*. Mea6/cTAGE5 cooperates with TRAPPC12 to regulate PTN secretion and white matter development. iScience. 2024;27(3):109180.

Yuting Feng, Shuyi Wang, Xiaoye Liu, Yiming Han, Hongwei Xu, Xiaocen Duan, Wenyue Xie, Zhuoling Tian, Zuoying Yuan, Zhuo Wan, Liang Xu, Siying Qin, Kangmin He, Jianyong Huang*. Geometric constraint-triggered collagen expression mediates bacterial-host adhesion. Nature Communications. 2023;14(1):8165.

Zhongsheng Wu^#, Yu Gao^#, Yongtao Du^#, Song Dang, Kangmin He*. The protocol of tagging endogenous proteins with fluorescent tags using CRISPR-Cas9 genome editing. Yi Chuan (Hereditas). 2023;45(2):165-175.

Chang Qiao^#, Di Li^#, Yong Liu^#, Siwei Zhang^#, Kan Liu, Chong Liu, Yuting Guo, Tao Jiang, Chuyu Fang, Nan Li, Yunmin Zeng, Kangmin He, Xueliang Zhu, Jennifer Lippincott-Schwartz*, Qionghai Dai*, Dong Li*. Rationalized deep learning super-resolution microscopy for sustained live imaging of rapid subcellular processes. Nature Biotechnology (2022) doi: 10.1038/s41587-022-01471-3.

Yi-Ying Chou^#, Srigokul Upadhyayula^#, Justin Houser, Kangmin He, Wesley Skillern, Gustavo Scanavachi, Song Dang, Anwesha Sanyal, Kazuka G. Ohashi, Giuseppe Di Caprio, Alex J.B. Kreutberger, Tegy John Vadakkan, Tom Kirchhausen*. Inherited nuclear pore substructures template post-mitotic pore assembly. Developmental Cell (2021) 56:1786-1803.e9.

Frederick B Shipley^#, Neil Dani^#, Huixin Xu, Christopher Deister, Jin Cui, Joshua P Head, Cameron Sadegh, Ryann M Fame, Morgan L Shannon, Vanessa I Flores, Thomas Kishkovich, Emily Jang, Eric M Klein, Glenn J Goldey, Kangmin He, Yong Zhang, Michael J Holtzman, Tomas Kirchhausen, Claire Wyart, Christopher I Moore, Mark L Andermann*, Maria K Lehtinen*. Tracking Calcium Dynamics and Immune Surveillance at the Choroid Plexus Blood-Cerebrospinal Fluid Interface. Neuron (2020) S0896-6273(20)30655-3.

Mingliang Zhang^#, Zhen Zhang^#, Kangmin He, Jimin Wu, Nan Li, Rong Zhao, Jinghe Yuan, Han Xiao, Youyi Zhang*, Xiaohong Fang*. Quantitative Characterization of the Membrane Dynamics of Newly Delivered TGF-β Receptors by Single-Molecule Imaging. Analytical Chemistry (2018) 90:4282-4287.

Jing Shen^#, Jianghui Zhang^#, Han Xiao^#, Jimin Wu, Kangmin He, Zhizhen Lv, Zijian Li, Ming Xu, Youyi Zhang*. Mitochondria are transported along microtubules in membrane nanotubes to rescue distressed cardiomyocytes from apoptosis. Cell Death & Disease (2018) 9:81.

Mingliang Zhang, Kangmin He, Jimin Wu, Nan Li, Jinghe Yuan, Wei Zhou, Zi Ye, Zijian Li, Zhizhen Lv, Xiaohong Fang*, Youyi Zhang*. Single-molecule imaging reveals the stoichiometry change of epidermal growth factor receptor during transactivation by β2-adrenergic receptor. SCIENCE CHINA Chemistry (2017) 60:1310-1317.

Yu Hu^#, Ji Hyung Kim^#, Kangmin He, Qi Wan, Jessica Kim, Melanie Flach, Tom Kirchhausen, Andrea Vortkamp, Florian Winau*. Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion. Journal of Experimental Medicine (2016) 213:2759-2772.

Francois Aguet^#, Srigokul Upadhyayula^#, Raphael Gaudin^#, Yi-ying Chou, Emanuele Cocucci, Kangmin He, Bi-Chang Chen, Kishore Mosaliganti, Mithun Pasham, Wesley Skillern, Wesley R. Legant, Tsung-Li Liu, Greg Findlay, Eric Marino, Gaudenz Danuser, Sean Megason, Eric Betzig, Tom Kirchhausen*. Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy. Molecular Biology of the Cell (2016) 27:3418-3435.

Yi-ying Chou, Christian Cuevas, Margot Carocci, Sarah H. Stubbs, Minghe Ma, David K. Cureton, Luke Chao, Frances Evesson, Kangmin He, Priscilla L. Yang, Sean P. Whelan, Susan R. Ross, Tom Kirchhausen*, Raphael Gaudin*. Identification and characterization of a novel broad-spectrum virus entry inhibitor. Journal of Virology (2016) 90:4494-4510.

Kangmin He, Rui Xing, Xiaohua Yan, Aiju Tian, Mingliang Zhang, Jinghe Yuan, Zhizhen Lv, Xiaohong Fang, Zijian Li*, Youyi Zhang*. mAbp1/HIP-55 is essential for the scission of clathrin-coated pits by regulating dynamin-actin interaction. FASEB Journal (2015) 29:2495-503.

Jinghe Yuan*, Kangmin He, Ming Cheng, Jianqiang Yu, Xiaohong Fang*. Analysis of the steps in single-molecule photobleaching traces by using the hidden markov model and maximum likelihood clustering. Chemistry – An Asian Journal (2014) 9:2303-2308.

Wangxi Luo, Kangmin He, Tie Xia, Xiaohong Fang*. Single-molecule monitoring in living cells using fluorescence microscopy. Analytical and Bioanalytical Chemistry (2013) 405:43-49.

Kangmin He, Xiaoli Shi, Xuejie Zhang, Song Dang, Xiaowei Ma, Fei Liu, Ming Xu, Zhizheng Lv, Dong Han, Xiaohong Fang*, Youyi Zhang*. Long-distance intercellular connectivity between cardiomyocytes and cardiofibroblasts mediated by membrane nanotubes. Cardiovascular Research (2011) 92:39–47.

Kangmin He^#, Wangxi Luo^#, Yuliang Zhang, Fei Liu, Da Liu, Li Xu, Lei Qin, Chunyang Xiong, Zhizhen Lu, Xiaohong Fang*, Youyi Zhang*. Intercellular transportation of quantum dots mediated by membrane nanotubes. ACS nano (2010) 4:3015-3022.